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0.4% trypan blue staining solution  (Thermo Fisher)


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    Thermo Fisher 0.4% trypan blue staining solution
    0.4% Trypan Blue Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/0.4% trypan blue staining solution/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    0.4% trypan blue staining solution - by Bioz Stars, 2026-03
    90/100 stars

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    The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
    0.4% Trypan Blue Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
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    Image Search Results


    The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were stained with trypan blue at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.

    Journal: Frontiers in Microbiology

    Article Title: RmCP, a cerato-platanin protein from Rigidoporus microporus , induces defense responses during interaction with Hevea brasiliensis

    doi: 10.3389/fmicb.2025.1553350

    Figure Lengend Snippet: The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were stained with trypan blue at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.

    Article Snippet: Leaf tissues were immersed in 0.5% trypan blue stain solution (Nacalai Tesque, Japan) for an hour followed by rinsing in absolute ethanol.

    Techniques: Activity Assay, Control, Staining, Light Microscopy